Fig. 1

Expression level of AMIGO2 in bladder cancer cells and tissues. A mRNA expression of AMIGO2 in one normal bladder epithelial cell line SV-HUC-1 and two bladder cancer cell lines: T24 and 5637. B The mRNA expression level of AMIGO2 was examined in cancer tissues (T) and adjacent normal tissues (ANT) (n = 11). C Representative HE and IHC images of AMIGO2 protein expression in cancer tissues and the corresponding adjacent normal tissues (n = 16). All images were captured at 50 × and 100 × magnification. D The violin plots of AMIGO2 protein expression level in all 16 pairs of tissue samples. Data were based on at least three independent experiments, and shown as mean ± SEM (*p < 0.05, ** p < 0.01, *** p < 0.001). BLCA: Bladder Cancer. TMA: Tissue Microarray