Fig. 6

Overexpression of PPAR-γ reverted the inhibitory effect of AMIGO2 in bladder cancer cells. Experimental grouping: shAMIGO2 + PPAR-γ and Negative Control. A The expression level of PPAR-γ in untreated T24, 5637 and SV-HUC-1 cell lines; the expression level of PPAR-γ in shAMIGO2 + PPAR-γ and Negative Control group of bladder cancer cell lines T24 and 5637. Measured by Western Blot. β-Actin served as the loading control. B Overexpression of PPAR-γ reverted the inhibitory effect of AMIGO2 on proliferation of bladder cancer cells, detected by CCK-8. C Overexpression of PPAR-γ reverted the inhibitory effect of AMIGO2 on migration of bladder cancer cells, assessed by wound healing assay. D Overexpression of PPAR-γ reverted the inhibitory effect of AMIGO2 on cell cycle of bladder cancer cells, analyzed by flow cytometry