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Fig. 2 | Hereditas

Fig. 2

From: HOXD9/APOC1 axis promotes macrophage M1 polarization to exacerbate diabetic kidney disease progression through activating NF-κB signaling pathway

Fig. 2

APOC1-enriched exosomes originating from HG-treated HPC promoted M1 macrophage activation. The experiment was divided into NG-exos, HG-exos, and sh-APOC1 + HG-exos groups (A) Flow cytometric analysis of THP-1 cells. (B) Fluorescence staining of PKH67-labeled HPC exosomes and DAPI-labeled THP-1 M0 cells. (C-D) Western blot analysis of iNOS, TNF-α, Arg-1, and CD206. (E-F) The mRNA expressions of iNOS, TNF-α, Arg-1, and CD206. (G-H) Detection of HPC exosomes and THP-1 M0 cells co-cultured chemotaxis by wound healing assay and Transwell invasion assay. (I) The mRNA expressions analysis of IL-6, NOS2, IL-1β, and MCP-1 by qPCR. The values in the graphs represent the mean ± SD. * P < 0.05; ** P < 0.01, *** P < 0.001

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Hereditas

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