Fig. 10
From: CDCA genes as prognostic and therapeutic targets in Colon adenocarcinoma
CDCA2 knockdown reduces proliferation and colony formation while enhancing migration in SW480 cells. (A) CDCA2 mRNA levels were significantly reduced in SW480 cells transfected with CDCA2-targeting siRNA (si-CDCA2-SW480) compared to control cells transfected with non-targeting siRNA (Ctrl-SW480). (B) Proliferation rates, measured by cell viability assays, were markedly decreased in si-CDCA2-SW480 cells relative to control cells. (C) The number of colonies formed was significantly lower in si-CDCA2-SW480 cells, indicating impaired growth and survival capabilities. (D) Crystal violet staining of the wound healing assay at 24 h shows enhanced wound closure in si-CDCA2-SW480 cells compared to control cells. (E) The percentage of wound closure was significantly higher in si-CDCA2-SW480 cells. (F) Microscopy images at 0 h and 24 h demonstrate faster wound closure in si-CDCA2-SW480 cells. (G) Graph depicting the percentage of wound closure over 24 h, showing a faster closure rate in si-CDCA2-SW480 cells. P*-value < 0.05 was considered statistically significant