Fig. 2

Protein-protein interaction (PPI) network analysis of coexistent targets, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis for gene targets of JWQZG in the treatment of NAFLD. (A) PPI network of 43 key genes identified using Degree Centrality (DC), Betweenness Centrality (BC), and Closeness Centrality (CC). (B) Network of the top 10 gene targets ranked by Degree values. Circle nodes represent key gene targets, with node size and color (ranging from yellow to red) reflecting their Degree values, transitioning from low to high. (C) GO enrichment analysis depicting the top 10 terms for BP, MF, and CC. The x-axis represents the number of enriched genes, and the y-axis lists the GO terms. (D) KEGG pathway enrichment analysis revealing the top 15 pathways for gene targets of JWQZG. Node size represents the count of enriched genes, while node color reflects the statistical significance (p-values). (E) Molecular docking of AKT1 (AF_P31749 ) with Berberine (affinity=-8.8 kcal/mol), Paeoniflorin (affinity=-8.5 kcal/mol), Quercetin (affinity=-8.4 kcal/mol), Wuweizisu C (affinity=-7.3 kcal/mol). (f) Molecular docking of IRS1 (AF_P35568 ) with Berberine (affinity=-6.2 kcal/mol), Paeoniflorin (affinity=-6.2 kcal/mol), Quercetin (affinity=-6.5 kcal/mol), Wuweizisu C (affinity=-6.4 kcal/mol).JWQZG: Jiu Wei Qing Zhi Gao