Fig. 2

Knockdown of Circ-MBOAT2 inhibits NSCLC cell proliferation, EMT and immune escape A: RT-qPCR to detect circ-MBOAT2; B-C: CCK8 assay and EdU assay to detect cell proliferation; D: AnnexinV-PI double staining to detect apoptosis rate; E: Western blot to detect the protein expression level of cellular Ki-67, Bax, and Bcl-2; F: Transwell assay to detect cell migration and invasion; G: Western blot to detect protein expression levels of cellular E-cadherin, N-cadherin and Vimentin; H: Flow cytometry detection of the percentage of CD8⁺ T cells in PBMCs; I: ELISA detection of TNF-α, IFN-γ, and IL-10 in the supernatant of PBMCs; J: Comparison of transplanted tumors volumes and weights in nude mice (N = 5); K: Immunohistochemical assessment of Ki67, E-cadherin and N-cadherin in nude mice transplanted tumor tissues (N = 5). * P < 0.05. ns, not significant. Each experiment in this work was conducted independently at least three times