Fig. 6
From: RNA-binding protein gene NOP58 exhibits crucial prognostic and therapeutic value in Ewing sarcoma
Knockdown of NOP58 influenced the proliferation of EwS cells. (A, B). An immunofluorescence assay was performed to detect the localization of the NOP58 protein in SK-N-MC and A673 cells using microscopy. (C, D). SK-N-MC and A673 cells were transfected with sh Ctrl, sh1 NOP58 and sh2 NOP58. Western blot and qRT-PCR assays were conducted to evaluate NOP58 expression in transfected cells. (E, F). CCK-8 assay was performed to assess cell proliferation. *** meant p was smaller or equaled 0.001; **** meant p was smaller or equaled 0.0001