Fig. 3

PTGS2 is a potential target of CUR. A The expression of PTGS2 was detected when WI-38 cells were treated with the LPS or combined with different concentrations of CUR. * P < 0.05 and *** P < 0.001 by one-way ANOVA and Tukey’s test. B The structure of CUR. C Biomolecular interaction of CUR and PTGS2 predicted by PyMOL system. The blue lines represent hydrogen bonds, and the yellow dash lines represent hydrophobic actions. D The 2D schematic diagram. On the left side, from top to bottom, were van der Waals, conventional Hydrogen Bond, and Carbon Hydrogen Bond; the right side represented Alky1 and Pi-Alky1 interactions from top to bottom. E The expression of PTGS2 mRNA was determined when WI-38 cells were treated with the LPS or combined with different concentrations of CUR. *** P < 0.001 by one way ANOVA and Tukey’s test, and ns means no significant difference. F After WI-38 cells were stimulated with CUR (7.5 µM), the CHX (25 µg/mL) was performed to treat the cells for 0, 6, 12, or 24 h. The PTGS2 protein expression was examined using western blot. * P < 0.05 and *** P < 0.001 by two-way ANOVA and Tukey’s test. Fig. S1: Biomolecular interaction of PTGS2 inhibitor celecoxib and PTGS2 predicted by PyMOL system