Fig. 4
From: Curcumin alleviates LPS-induced WI-38 cell inflammation injury by regulating PTGS2 expression
Knockdown of PTGS2 alleviates the LPS-stimulated WI-38 cell inflammation damage. WI-38 cells were transfected with si-NC or si-PTGS2 followed by treatment with 10 μg/mL LPS. A The PTGS2 expression was detected by western blot. B CCK8 was used to test the cell vitality. C The cell proliferation was tested by an EdU assay. D The ability of apoptosis was examined using flow cytometry. E–G The corresponding test kits were carried out to examine the inflammation factor productions. H The MDA levels were analyzed using the MDA assay kit. I The SOD activity assay kit was used to detect the level of SOD. ** P < 0.01 and *** P < 0.001 by one-way ANOVA and Tukey’s test