Fig. 6

Circ-RPPH1 regulates EMT-related proteins by preventing EIF4 A3 from recruiting their respective mRNA. A RT-qPCR detection of circ-RPPH1 in T24 cells transfected with overexpressed vector. B-C. RIP detected EMT-related protein mRNA enrichment in EIF4 A3 RNA after circ-RPPH1 overexpression. D-E Immunoblotting analysis of EMT-related proteins. F Immunoblotting analysis of EMT protein after EIF4 A3 was down-regulated. Note: all experiment independently repeated three times, * P < 0.05, P < 0.01, P * * * * * < P < 0.0001, 0.001, * * * *